microrna-297a regulates vascular calcification by targeting fibroblast growth factor 23
نویسندگان
چکیده
objective(s): vascular calcification is one the major characteristics in patients with various types of chronic inflammatory disorders. mirnas have been shown to be involved in many normal biological functions as well as diseases; however, their role in vascular calcification has not received much attention. materials and methods: in the current study, we built a vascular calcification rat model using vitamin d3 plus nicotine and analyzed mirna expression profile by mirna chip assay. potential target of one selected mirna with sharpest variation in expression were predicted by both pictar and targetscan. the impact of the selected mirna on the expression of the potential target on both mrna and protein levels were measured by rt-pcr and western blot, respectively. results: our results identified 16 dysregulated mirnas, among which mir-297a showed the sharpest variation. further analysis focusing on mir-297a revealed that fibroblast growth factor 23 (fgf23) was a potential target of mir297a. measurement of fgf23 and its regulator klotho on both mrna and protein levels demonstrated that fgf23 was significantly increased while klotho was decreased in rats with vascular calcification. conclusion: our results indicated that fgf23 was target of mir-297a and decreased mir-297a in vascular calcification lead to the increase of fgf23, which together with klotho might enhance vascular calcification. the findings of this study could provide valuable information for the understanding of mechanisms underlying mir-dependent vascular calcification as well as potential treatment target for the disease.
منابع مشابه
MicroRNA-297a regulates vascular calcification by targeting fibroblast growth factor 23
Objective(s): Vascular calcification is one the major characteristics in patients with various types of chronic inflammatory disorders. MiRNAs have been shown to be involved in many normal biological functions as well as diseases; however, their role in vascular calcification has not received much attention. Materials and Methods: In the current study, we built a vascular calcification rat mode...
متن کاملMicroRNA-297a regulates vascular calcification by targeting fibroblast growth factor 23
OBJECTIVES Vascular calcification is one the major characteristics in patients with various types of chronic inflammatory disorders. MiRNAs have been shown to be involved in many normal biological functions as well as diseases; however, their role in vascular calcification has not received much attention. MATERIALS AND METHODS In the current study, we built a vascular calcification rat model ...
متن کاملMiR-103 regulates the angiogenesis of ischemic stroke rats by targeting vascular endothelial growth factor (VEGF)
Objective(s): To investigate the effect of miR-103 on the angiogenesis of ischemic stroke rats via targeting vascular endothelial growth factor (VEGF) at the molecular level. Materials and Methods: Rat models had received the middle cerebral artery occlusion (MCAO) or sham operation before grouping, and cell models of oxygen-glucose deprivation (OGD) were performed. FITC-dextran, matrigel, and ...
متن کاملThe Association between Fibroblast Growth Factor-23 and Vascular Calcification Is Mitigated by Inflammation Markers
BACKGROUND Fibroblast growth factor-23 (FGF-23) has been linked to vascular calcification, ventricular hypertrophy and mortality in chronic kidney disease (CKD), although these links may not be direct and independent. Similar grave outcomes are associated with inflammation and oxidative stress in CKD. Recently, accumulating evidence has linked components of phosphate homeostasis to inflammation...
متن کاملFibroblast Growth Factor 23 and Vascular Calcification: Is It Set in Stone.
health [5] . The volumetric bone mineral density, estimated glomerular filtration rate (eGFR), albuminuria and levels of calcified atherosclerotic plaque (CAP) in coronary, carotid and aortic vascular beds were correlated [5] . After adjusting for covariates, analyses revealed an expected negative correlation with eGFR. However, the core finding observed by the investigators was the association...
متن کاملVascular Medicine Vascular Klotho Deficiency Potentiates the Development of Human Artery Calcification and Mediates Resistance to Fibroblast Growth Factor 23
Received July 3, 2011; accepted March 9, 2012. From the Department of Medicine, Renal Division, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA (K.L., T.-S.L., C.L., L.-L.H.); Clinical Sciences Research Institute, Warwick Medical School (K.L., G.M., D.Z.); and University Hospital Coventry & Warwickshire NHS Trust, Coventry, United Kingdom (F.T.L., D.Z.). Drs Lim and Lu contribu...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of basic medical sciencesجلد ۱۹، شماره ۱۲، صفحات ۱۳۳۱-۱۳۳۶
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023